High TNT-transforming activity by a mixed culture acclimated and maintained on crude-oil-containing media

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DOIResolve DOI: http://doi.org/10.1139/W03-049
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TypeArticle
Journal titleCanadian Journal of Microbiology
Volume49
Issue5
Pages362366; # of pages: 5
Subjectenv; Cells; Nitrogen
AbstractA mixed microbial culture originating from a petroleum-contaminated site and maintained on crude oil exhibited high 2,4,6-trinitrotoluene (TNT) transformation activity. Cultivation of the mixed culture in glucose-containing medium for 29 h resulted in almost complete transformation of 100 ppm TNT. TNT transformation was observed with both growing and resting cells. With subculturing, it was found that TNT could support growth of the mixed culture when supplied as sole carbon source, sole nitrogen source, or sole carbon and nitrogen source. The finding that a mixed microbial culture maintained on crude oil exhibited high TNT transformation activity without prior subculture on TNT-containing media is novel and may have potential practical applications in the bioremediation of munitions-contaminated soil and wastewater.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number45944
NPARC number3539302
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Record identifierb134e141-579f-4a80-bbfd-23c5410383c9
Record created2009-03-01
Record modified2016-05-09
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