Nickel nanoparticles synthesized by a modified polyol method for the purification of histidine-tagged single-domain antibody ToxA5.1

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DOIResolve DOI: http://doi.org/10.1557/jmr.2012.323
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TypeArticle
Journal titleJournal of Materials Research
ISSN0884-2914
Volume27
Issue22
Pages28842890; # of pages: 7
SubjectAdsorption cycle; Average diameter; Binding capacities; Ferromagnetic behaviors; magnetic; Magnetic adsorbents; Magnetic characterization; Magnetization-saturation; Modified polyol method; Nickel nanoparticles; Nucleating agents; Palladium chloride; Polyvinyl pyrrolidone; Protective agents; Protein purification; Single domains; Adsorbents; Adsorption; Alcohols; Amino acids; Antibodies; Chlorine compounds; Ethylene glycol; Magnetic domains; Nanomagnetics; Nanoparticles; Nickel; Nickel compounds; Palladium; Purification; Recombinant proteins; Saturation magnetization; X ray diffraction; Biosynthesis
AbstractNickel nanoparticles (NNPs) synthesized by a modified polyol method using ethylene glycol as a reducing agent, palladium chloride as a nucleating agent, and polyvinylpyrrolidone (PVP) as a protective agent were investigated as a potential magnetic adsorbent for the purification of hexahistidine-tagged (His6-tagged) recombinant proteins. The synthesis resulted in nanoparticles having an average diameter of 6828 nm. The x-ray diffraction pattern confirmed the presence of nickel metal, as well as the presence of unreacted nickel (II) hydroxide Ni(OH)2. Magnetic characterization showed that a magnetization saturation of 39.3 electromagnetic unit (emu)/g at 20,000 Oersted (Oe) was reached rapidly and that the material exhibited ferromagnetic behavior. Protein purification results showed that the synthesized NNPs were highly selective for binding to a His6-tagged recombinant protein single-domain antibody ToxA5.1. In addition, NNPs were used for four adsorption cycles without significant binding capacity losses. These particles have shown great potential such as being easily synthesized, cost-effective, and highly selective magnetic adsorbents for the purification of His6-tagged recombinant proteins. © Copyright © Materials Research Society 2012.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21269398
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Record identifierb1c3d0e3-befa-4853-86c1-742479160b0e
Record created2013-12-12
Record modified2016-05-09
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