Shewanella halifaxensis sp. nov, a novel obligately respiratory and denitrifying psychrophile

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DOIResolve DOI: http://doi.org/10.1099/ijs.0.63829-0
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TypeArticle
Journal titleInternational Journal of Systematic and Evolutionary Microbiology (IJSEM)
Volume56
Issue1
Pages205212; # of pages: 8
AbstractIndigenous bacteria found in the sediment of the Emerald Basin (depth of 215 m, Atlantic Ocean) located offshore of Halifax Harbour (Nova Scotia, Canada) were previously found to be able to degrade the explosive compound hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). In the present study, a novel obligately respiratory, denitrifying and RDX-mineralizing bacterium, designated strain HAW-EB4T, was isolated from the marine sediment. This bacterium utilized peptone, yeast extract, Casamino acids, esters (Tweens 20, 40 and 80), sugars (N-acetyl-d-glucosamine, ribose), several C2 and C3 acids (acetate, pyruvate, lactate, propionate) and amino acids (serine, proline) as sole carbon and energy sources. Aerobically grown cells (in marine broth 2216 at 10 °C) contained C14 : 0 (6 %), iso-C15 : 0 (12 %), C16 : 0 (20 %), C16 : 1ω7 (37 %), C18 : 1ω7 (7 %) and C20 : 5ω3 (7 %) as major membrane fatty acids, and Q7 (28·1 %) and MK-7 (60·9 %) as dominant respiratory quinones, consistent with deep-sea species of Shewanella. The novel bacterium had a DNA G+C content of 45 mol% and showed similarity to Shewanella species in terms of 16S rRNA and gyrB gene sequences (93–99 and 67·3–88·4 % similarity, respectively), with Shewanella pealeana being the most closely related species. Genomic DNA–DNA hybridization between strain HAW-EB4T and S. pealeana revealed a level of relatedness of 17·9 %, lower than the 70 % species cut-off value, indicating that strain HAW-EB4T (=NCIMB 14093T=DSM 17350T) is the type strain of a novel species of Shewanella, for which the name Shewanella halifaxensis sp. nov. is proposed.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number47246
NPARC number3539382
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Record identifierb1cb68dc-80d4-4c98-8ec7-4c3af0bc2bf7
Record created2009-03-01
Record modified2016-05-09
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