Use of a dual-labelled oligonucleotide as a DNA dosemeter for radiological exposure detection

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DOIResolve DOI: http://doi.org/10.1093/rpd/ncq599
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleRadiation Protection Dosimetry
ISSN0144-8420
Volume148
Issue1
Article numberncq599
Pages2033; # of pages: 14
Subjectbioassay; equipment design; genetic procedures; instrumentation; occupational exposure; radiation dose; radiation exposure; radiation monitoring; reproducibility; sensitivity and specificity; spectrofluorometry; staining; Biological Assay; Biosensing Techniques; DNA; Equipment Design; Equipment Failure Analysis; Occupational Exposure; Radiation Dosage; Radiation Monitoring; Reproducibility of Results; Sensitivity and Specificity; Spectrometry, Fluorescence; Staining and Labeling
AbstractA reporter molecule consisting of a synthetic oligonucleotide is being characterised for a novel damage detection scenario for its potential use as a field-deployable, personal deoxyribonucleic acid (DNA) dosemeter for radiation detection. This dosemeter is devoid of any biological properties other than being naked DNA and therefore has no DNA repair capabilities. It supports biodosimetry techniques, which require lengthy analysis of cells from irradiated individuals, and improves upon inorganic dosimetry, thereby providing for a more relevant means of measuring the accumulated dose from a potentially mixed-radiation field. Radiation-induced single strand breaks (SSBs) within the DNA result in a quantifiable fluorescent signal. Proof of concept has been achieved over 250 mGy-10 Gy dose range in radiation fields from . 60Co, with similar results seen using a linear accelerator X-ray source. Further refinements to both the molecule and the exposure/detection platform are expected to lead to enhanced levels of detection for mixed-field radiological events. © Crown copyright 2011
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Medical Devices
Peer reviewedYes
NPARC number21270323
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Record identifierb22f9884-a059-4f3c-a575-2bd5370f40a5
Record created2014-01-24
Record modified2016-05-09
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