Assessment of cytotoxicity using electric cell-substrate impedance sensing (ECIS):Concentration and time response function approach

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DOIResolve DOI: http://doi.org/10.1021/ac025848f
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TypeArticle
Journal titleAnalytical Chemistry
Volume74
Issue22
Pages57485753; # of pages: 6
Subjectenv
AbstractThis paper describes a simple and convenient method to measure the concentration and time response function f (C,t) of cells exposed to a toxicant by electric cell-substrate impedance sensing. Attachment and spreading of fibroblastic V79 cells cultured on small gold electrodes precoated with fibronectin were detected as electrical resistance changes. With this method, chemical cytotoxicity was easily screened by observing the response function of attached cells in the presence of inhibitor. The cytotoxicities of three test models, cadmium chloride, sodium arsenate, and benzalkonium chloride, were quantified by measuring the percentage inhibition as a function of the inhibitor concentration. The half-inhibition concentration, the required concentration to achieve 50% inhibition, derived from the response function agreed well with the results obtained using the standard neutral red assay.
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number45891
NPARC number3538971
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Record identifierb2529cb4-d0d7-4a3e-8700-19e0372505a8
Record created2009-03-01
Record modified2016-05-09
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