Low-intensity pulsed ultrasound-mediated stimulation of hematopoietic stem/progenitor cell viability, proliferation and differentiation in vitro

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DOIResolve DOI: http://doi.org/10.1007/s10529-012-0984-6
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleBiotechnology Letters
ISSN0141-5492
1573-6776
Volume34
Issue10
Pages19651973; # of pages: 9
AbstractLow-intensity pulsed ultrasound (LIPUS) stimulated the viability, proliferation and differentiation of hematopoietic stem/progenitor cells (HSPC) from fresh and cryopreserved peripheral blood leukapheresis product, as well as cord blood when applied for 10 min each day for 4 days. Cell viability, proliferation and differentiation were assessed on day 5 by viable cell counting, MTS proliferation assay, flow cytometry, and colony-forming unit assay. LIPUS stimulation: (i) enhanced the proliferation of fresh HSPC and maintained the viability of cryopreserved HSPC in vitro; (ii) did not affect the percentage of CD34 + and CD14 + cells; and (iii) enhanced burst-forming unit-erythroid colony formation. Hence, we suggest that this novel LIPUS stimulation approach might enhance the efficacy of clinical transplantation and cellular therapies using HSPC. © 2012 Springer Science+Business Media B.V.
Publication date
LanguageEnglish
AffiliationSecurity and Disruptive Technologies; NRC Industrial Research Assistance Program; National Research Council Canada
Peer reviewedYes
NPARC number21270061
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Record identifierb285821f-285a-495d-ab55-42eeda01c774
Record created2013-12-18
Record modified2016-05-09
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