Antioxidant and co-antioxidant activity of vitamin C. The effect of vitamin C, either alone or in the presence of vitamin E or a water-soluble vitamin E analogue, upon the peroxidation of aqueous multilamellar phospholipid liposomes

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DOIResolve DOI: http://doi.org/10.1016/0005-2760(85)90285-1
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TypeArticle
Journal titleBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
ISSN0005-2760
Volume835
Issue2
Pages298303; # of pages: 6
Subject2,2' azobis(2 amidinopropane); alpha tocopherol; ascorbic acid; lipid peroxide; liposome; trolox c; 2,2' azobis(2,4 dimethylvaleronitrile); dilinoleoylphosphatidylcholine; dose response; drug comparison; drug interaction; drug mechanism; drug potentiation; drug response; nonbiological model; nonhuman; priority journal; theoretical study; Antioxidants; Ascorbic Acid; Benzopyrans; Chromans; Kinetics; Liposomes; Models, Biological; Oxidation-Reduction; Oxygen Consumption; Phosphatidylcholines; Support, Non-U.S. Gov't; Vitamin E
AbstractThermally labile azo-initiators, dissolved in either the aqueous or lipid phase, have been used to generate peroxyl radicals at a known, steady rate in an aqueous dispersion of dilinoleoylphosphatidylcholine multilamellar liposomes at 37° C in order to study the antioxidant behaviour of ascorbate itself and ascorbate in combination with either α-tocopherol or a water-soluble α-tocopherol analogue (TROLOX(-)). It is found that ascorbate is an effective inhibitor of peroxidations initiated in the aqueous phase, with each ascorbate terminating 0.6 radical chains (i.e., n = 0.6), but it is a very poor inhibitor of peroxidations initiated in the lipid phase. Peroxidations initiated in the lipid-phase in the presence of either α-tocopherol or TROLOX(-) indicate that ascorbate is an excellent synergist with both phenolic antioxidants (n = 0.4). In peroxidations initiated in the aqueous phase ascorbate acts as a co-antioxidant with TROLOX(-) (n = 0.7), but the interpretation of the approximately additive effect obtained in the presence of α-tocopherol is complicated by the fact that under the experimental conditions employed α-tocopherol alone does not give a distinct, measurable inhibition period. The latter problem is shown to be due to a non-uniform distribution of the water-soluble initiator within the liposome. Other examples of the complicating effects of non-uniform distributions of reactants in kinetic studies of the autoxidation of organic substrates dispersed in water are described. © 1985.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21276482
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Record identifierb3dd36ba-2cdb-48ee-96fe-8a67bc088841
Record created2015-10-13
Record modified2016-05-09
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