Infrared spectroscopic studies of Acholeplasma laidlawii B membranes : comparison of the gel to liquid-crystal phase transition in intact cells and isolated membranes

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DOIResolve DOI: http://doi.org/10.1111/j.1432-1033.1988.tb14455.x
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TypeArticle
Journal titleEuropean Journal of Biochemistry
ISSN1742-4658
Volume178
Issue2
Pages335341; # of pages: 7
AbstractThe physical state of the membrane lipids in the plasma membranes of intact, live Acholeplasma laidlawii B cells was probed by Fourier-transform infrared spectroscopy and compared with that in isolated membranes. Infrared spectra of live A. laidlawii B cells, enriched biosynthetically in the presence of avidin, with saturated deuterated and unsaturated non-deuterated fatty acids have been recorded at a variety of temperatures. The results indicate that within the temperature range of the gel to liquid-crystal phase transition, the live cells are able to keep the ‘fluidity’ of their plasma membranes at a considerably higher value compared to that in the isolated membranes at the same temperature. While this is a generally valid observation, the degree by which live and isolated membranes differ in their liquid-crystal-phase content at a given temperature depends on the nature of the exogenous fatty acid and the temperature of growth.
Publication date
PublisherJohn Wiley & Sons, Inc.
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NRC numberMANTSCH1988
NPARC number9386959
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Record identifierb47a4a70-78de-41ce-9946-06c8abe6c597
Record created2009-07-10
Record modified2016-12-13
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