Structure of the lipid A of Bordetella hinzii ATCC 51730: Rapid Commun.Mass Spectrom.

  1. Get@NRC: Structure of the lipid A of Bordetella hinzii ATCC 51730: Rapid Commun.Mass Spectrom. (Opens in a new window)
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Journal titleRapid Commun.Mass Spectrom.
Pages595599; # of pages: 5
SubjectACID; Acids; Acylation; amide; analysis; Bordetella; Bordetella bronchiseptica; Bordetella pertussis; Carbohydrate Conformation; chemical; chemistry; Chromatography,Gel; Chromatography,Thin Layer; Comparative Study; COMPONENT; COMPONENTS; Desorption Mass Spectrometry; DISACCHARIDE; Electrophoresis,Polyacrylamide Gel; endotoxin; Esters; Fatty Acids; Glucosamine; Human; isolation & purification; laser; LINKAGES; lipid; Lipid A; LIPID-A; LIPOPOLYSACCHARIDE; Lipopolysaccharides; liquid chromatography/mass spectrometry; MAGNETIC; Magnetic Resonance Spectroscopy; Mass Fragmentography; mass spectrometry; MASS-SPECTROMETRY; Methods; Models,Molecular; MOLECULAR; NMR; NUCLEAR; Parapertussis; plasma; Silicon Dioxide; Species Specificity; Spectrometry,Mass,Matrix-Assisted Laser Desorption-Ionization; Spectrum Analysis,Mass; structure; Support,Non-U.S.Gov't
AbstractBordetella hinzii has recently been isolated from immunocompromised human hosts. The structure of the lipid A of its endotoxin was investigated using chemical analyses, nuclear magnetic resonnance (NMR), gas liquid chromatography/mass spectrometry (GC/MS), plasma desorption mass spectrometry (PDMS) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The lipid A contains the classical bisphosphorylated beta-(1-->6)-linked D-glucosamine disaccharide with hydroxytetradecanoic acid (C14OH) in amide linkages. The lipid A components of B. pertussis, B. bronchiseptica, and B. parapertussis all differ in their acylation pattern but share a residue of tetradecanoyl-3-hydroxytetradecanoic acid in amide linkage at the C-2' position. However, in the B. hinzii species, the tetradecanoic acid (C14) is stoichiometrically replaced by a 2-hydroxytetradecanoic acid (2-C14OH). In the few reported examples of a hydroxylated fatty acid in this position, the substitutions were only partial. The B. hinzii lipid A differs from that of B. pertussis also by replacement of the hydroxydecanoic acid (C10OH) by hydroxydodecanoic acid (C12OH) and by the presence of a hexadecanoic acid (C16) to give a sixth fatty acid. The lipid A was heterogeneous, being composed of three major molecular species: tetra-, penta- and hexaacylated. The fatty acids in ester linkage were localized by PDMS of the native and alkali-treated lipid A. The lipid A components isolated from the O-chain-linked lipopolysaccharides (LPSs) were shown to be more acylated than those from the O-chain-free LPSs
Publication date
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberAUSSEL2000B
NPARC number9378450
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Record identifierb49bf9ee-30c7-43ce-ba51-15752da83cb9
Record created2009-07-10
Record modified2016-05-09
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