Triploid and diploid rainbow trout do not differ in their stress response to transportation

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DOIResolve DOI: http://doi.org/10.1577/A05-035.1
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TypeArticle
Journal titleNorth American Journal of Aquaculture
Volume68
Issue1
Pages18; # of pages: 8
SubjectBiological stress; Coenzymes; Containment; Cytochemistry; Diploids; Endocrinology; Fish culture; Fish handling; Fish physiology; Freshwater aquaculture; Glucose; Hybrid culture; Neurophysiology; Polyploids; Selective breeding; Stocking density; Tanks; Transportation; Oncorhynchus mykiss; Canada; British Columbia
AbstractWe examined the neuroendocrine and cellular stress responses of diploid and triploid rainbow trout Oncorhynchus mykiss to transportation. Juvenile diploid and triploid rainbow trout (28 and 26 g/fish average weight, respectively) were stocked at 100 g/L in replicate 70-L tanks and subjected to transportation for an 8-h period. Subsequent levels of plasma cortisol and glucose and of cellular hepatic glutathione (GSH) and heat shock protein 70 (Hsp70) were similar between ploidy groups, indicating that triploid fish respond to transportation in much the same way as diploid fish. A stationary treatment was also included that involved confinement of experimental fish in similar tanks without transport to determine to what extent high-density containment contributed to the stress response in the absence of the noise and vibration of transport. Unexpectedly, fish in the stationary treatment had significantly higher plasma cortisol and glucose levels than the transported fish; however, this might be attributable to a confounding effect of hyperoxia, as oxygen levels fluctuated between 150% and 460% saturation in the stationary tank, while those in the transported tank remained within 100–200% saturation. We suggest that when long stops are necessary while transporting fish, water agitators be used to preclude the additional stress of excessive gas saturation. This may be particularly important for triploid fish, which had lower hepatic GSH levels than diploid fish as well as a low level of mortality in the stationary treatment, unlike the diploid fish.
Publication date
Copyright noticeCopyright by the American Fisheries Society 2006
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NRC number1553
NPARC number3538043
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Record identifierb4d551ad-3a58-480c-a35f-a6dd4bd69150
Record created2009-03-01
Record modified2016-05-09
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