Involvement of phosphate-modified ATP analogs in the reactions of oxidative phosphorylation

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DOIResolve DOI: http://doi.org/10.1016/0005-2728(79)90017-3
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TypeArticle
Journal titleBiochimica et Biophysica Acta (BBA) - Bioenergetics
ISSN0005-2728
Volume547
Issue2
Pages361369
SubjectOxidative phosphorylation; Respiration; ATPase; Nucleotide analog; (Mitochondria)
AbstractVarious analogs of adenosine 5′-triphosphate with a modified terminal phosphate group have been tested in energy-requiring reactions with intact mitochondria and submitochondrial particles. It is shown that the fluorophosphate analog ATP(γF) is a strong inhibitor of mitochondrial respiration and of energy requiring reactions which involve the participation of high energy intermediates, generated aerobically by the respiratory chain. On the other hand, ATP(γF) does not affect the ATPase activity of intact or disrupted mitochondria and is less effective in inhibiting ATP-driven reactions. The imidophosphate analog AMP-P(NH)P also inhibits the partial reactions of oxidative phosphorylation, but does not affect ATP synthesis from ADP and Pi. In contrast to ATP(γF), it is a strong inhibitor of both soluble and membrane-bound mitochondrial ATPases. The biological implication of the complementary effects of ATP(γF) and AMP-P(NH)P on mitochondria-catalysed reactions is discussed while suggesting the use of such nucleotide analogs as specific tools for the study of ATP-forming and ATP-utilizing reactions in mitochondria.
Publication date
PublisherElsevier B.V.
LanguageEnglish
AffiliationNational Research Council Canada
Peer reviewedYes
NPARC number23001277
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Record identifierb5a90073-e9c3-44c7-927c-b4edf662ea86
Record created2017-01-13
Record modified2017-01-13
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