Pretreating mixed anaerobic communities from different sources : correlating the hydrogen yield with hydrogenase activity and microbial diversity

Download
  1. Get@NRC: Pretreating mixed anaerobic communities from different sources : correlating the hydrogen yield with hydrogenase activity and microbial diversity (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1016/j.ijhydene.2012.05.105
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleInternational Journal of Hydrogen Energy
ISSN03603199
Volume37
Issue17
Pages1217512186; # of pages: 12
SubjectMixed anaerobic cultures; hydrogen; hydrogenase activity; T-RFLP; microbial diversity; pretreatment
AbstractIn this study, granular and flocculated anaerobic mixed cultures were pretreated using heat, shock loading, acid, alkali, linoleic acid (LA) and 2-bromoethane sulphonic acid (BESA). Under mesophilic conditions (37°C) and an initial pH value of 6.0, higher H₂ yields were observed for the flocculated cultures when compared to the granular cultures. The maximum yield for granular cultures treated acid, BESA or LA were statistically the same. Butyric acid fermentation was dominant in a majority of the treated cultures. The maximum hydrogenase evolution specific activity (ESA) (124 ± 8 Ue mg VSS⁻¹) at 37°C correlated with the maximum H₂ yield for the LA treated flocculated cultures (1.69 ± 0.18 mol mol⁻¹ glucose). The microbial diversity data clearly showed that the low H₂ yield in the granular cultures was due to the lower proportion of H₂ producers. A principle component analysis (PCA) revealed that the LA treated flocculated and granular cultures were grouped together and showed more diversity in comparison to other pretreatment methods.
Publication date
PublisherElsevier
LanguageEnglish
AffiliationNational Research Council Canada; Aquatic and Crop Resource Development
Peer reviewedYes
NRC number55025
NPARC number21268454
Export citationExport as RIS
Report a correctionReport a correction
Record identifierb5dc6c4a-4f33-4012-9052-7014dfe889fa
Record created2013-07-24
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)
Date modified: