Identification and characterization of interactions between abscisic acid and mitochondrial adenine nucleotide translocators

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DOIResolve DOI: http://doi.org/10.1042/BJ20101898
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TypeArticle
Journal titleBiochemical Journal
Volume437
Pages117123; # of pages: 7
Subjectphytohormone; mitochondria; adenine nucleotide translocator; inhibition; uptake
AbstractAbscisic acid (ABA) is a plant hormone involved in important processes including development and stress responses. Recent reports have identified a number of plant ABA receptors and transporters, highlighting novel mechanisms of ABA action. Herein we describe application of a chemical proteomics approach leading to the identification of mitochondrial adenine nucleotide translocators (ANTs) as ABA-interacting proteins. Initial in vitro studies confirmed inhibition of ANT-dependent ATP translocation by ABA. Further analysis demonstrated ANT-dependent uptake of ABA into both recombinant Arabidopsis thaliana ANT2-containing proteoliposomes and native isolated spinach mitochondria; the latter with a Km of 3.5 µM and a Vmax of 2.5 nmol/min/g protein. ATP was found to inhibit ANT-dependent ABA-translocation. Specificity profiles highlight the possibility of mechanistic differences in translocation of ABA and ATP. Finally, ABA was shown to stimulate ATPase activity in spinach mitochondrial extracts. ABA concentrations in plant cells are estimated to reach the low µM range during stress responses, supporting potential physiological relevance of these in vitro findings. Overall, this in vitro work suggests the possibility of as yet uncharacterized mechanisms of ABA action in planta related to inhibition of mitochondrial ATP-translocation and functional localization of ABA in the mitochondrial matrix.
Publication date
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
Peer reviewedYes
NRC number50170
NPARC number18786625
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Record identifierb68989bf-3d2f-4402-929d-00525b5e250d
Record created2012-04-17
Record modified2017-03-23
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