Genome-wide analysis reveals gene expression and metabolic network dynamics during embryo development in Arabidopsis

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DOIResolve DOI: http://doi.org/10.1104/pp.110.171702
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TypeArticle
Journal titlePlant Physiology
Volume156
Issue1
Pages346356; # of pages: 11
AbstractEmbryogenesis is central to the life cycle of most plant species. Despite its importance, because of the difficulty associated with embryo isolation, global gene expression programs involved in plant embryogenesis - especially the early events following fertilization are largely unknown. To address this gap, we have developed methods to isolate whole live Arabidopsis thaliana embryos as young as zygote and performed genome-wide profiling of gene expression. These studies revealed insights into patterns of gene expression relating to: maternal and paternal contributions to zygote development; chromosomal level clustering of temporal expression in embryogenesis; and embryo-specific functions. Functional analysis of some of the modulated transcription factor encoding genes from our datasets confirmed that they are critical for embryogenesis. Furthermore, we constructed stage-specific metabolic networks mapped with differentially regulated genes by combining the microarray data with the available KEGG metabolic datasets. Comparative analysis of these networks revealed the network-associated structural and topological features, pathway interactions and gene expression with reference to the metabolic activities during embryogenesis. Together, these studies have generated comprehensive gene expression datasets for embryo development in Arabidopsis and may serve as an important foundational resource for other seed plants.
Publication date
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; NRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC number50156
NPARC number18749434
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Record identifierb6a30894-00c3-486a-a932-d6f0a88eca00
Record created2012-03-30
Record modified2016-05-09
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