SST2, a regulator of G-protein signaling for the Candida albicans mating response pathway

Download
  1. Get@NRC: SST2, a regulator of G-protein signaling for the Candida albicans mating response pathway (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1128/EC.5.1.192-202.2006
AuthorSearch for: ; Search for:
TypeArticle
Journal titleEukaryotic Cell
Volume5
Issue1
Pages192202; # of pages: 11
SubjectCandida; Candida albicans; Cell Cycle; Cells; Gene Expression; pha; Protein; Saccharomyces cerevisiae
AbstractCandida albicans contains a functional mating response pathway that is similar to the well-studied system of Saccharomyces cerevisiae. We have characterized a regulator of G protein signaling (RGS) homolog in C. albicans with sequence similarity to the SST2 gene of Saccharomyces cerevisiae. Disruption of this gene, which had been designated SST2, causes an opaque MTLa/MTLa derivative of strain SC5314 to show hypersensitivity to the C. albicans alpha-factor. This hypersensitivity generates an enhanced cell cycle arrest detected in halo assays but reduces the overall mating efficiency of the cells. Transcriptional profiling of the pheromone-regulated gene expression in the sst2 mutant shows a pattern of gene induction similar to that observed in wild-type cells, but the responsiveness is heightened. This involvement of an RGS in the sensitivity to pheromone is consistent with the prediction that the mating response pathway in C. albicans requires the activation of a heterotrimeric G protein.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
NoteEnglish16400182
Peer reviewedNo
NRC number47485
NPARC number3538671
Export citationExport as RIS
Report a correctionReport a correction
Record identifierb6bfcf10-c427-44bf-b3fd-cf32dadc924c
Record created2009-03-01
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)