Perfluorophenyl azide immobilization chemistry for single molecule force spectroscopy of the concanavalin A/mannose interaction

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DOIResolve DOI: http://doi.org/10.1021/la1036579
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TypeArticle
Journal titleLangmuir
Volume26
Issue22
Pages1667716680; # of pages: 4
AbstractThe versatility of perfluorophenyl azide (PFPA) derivatives makes them useful for attaching a wide variety of biomolecules and polymers to surfaces. Herein, a single molecule force spectroscopy (SMFS) study of the concanavalin A/mannose interaction was carried out using PFPA immobilization chemistry. SMFS of the concanavalin A/mannose interaction yielded an average unbinding force of 70-80 pN for loading rates between 8000 and 40 000 pN/s for mannose surfaces on aminated glass, and an unbinding force of 57 ( 20 pN at 6960 pN/s for mannose surfaces on gold-coated glass. Dynamic force spectroscopy was used to determine the dissociation rate constant, koff, for this interaction to be 0.16 s-1.
Publication date
PublisherAmerican Chemical Society
LanguageEnglish
AffiliationNational Research Council Canada; NRC Steacie Institute for Molecular Sciences
Peer reviewedYes
NPARC number17364202
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Record identifierb6ee7993-ab8a-4a9b-a4f1-4796d676f54d
Record created2011-03-22
Record modified2016-05-09
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