Liquid chromatography post-column oxidation (PCOX) method for the determination of paralytic shellfish toxins in mussels, clams, oysters, and scallops: Collaborative study

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Journal titleJournal of AOAC International
Pages11541176; # of pages: 23
SubjectBiological methods; Blue mussels; Certified reference materials; Chromatographic methods; Crassostrea; Edible tissue; Fluorescence detection; Instrumental techniques; Method of analysis; Mouse bioassay; Mytilus edulis; Paralytic shellfish toxins; Performance parameters; Post-column; Reference method; Regulatory authorities; Relative standard deviations; Reproducibilities; Reversed-phase liquid chromatography; Soft shell clams; Test materials; Bioassay; Chromatographic analysis; Liquid chromatography; Metabolites; Oxidation; Tissue; Toxicity; Shellfish; Bivalvia; Crassostrea; Mya; Mya arenaria; Mytilus edulis; Ostreidae; Pectinidae; Placopecten magellanicus; marine toxin; animal; article; bivalve; chemistry; food contamination; isolation and purification; liquid chromatography; methodology; mouse; oxidation reduction reaction; shellfish poisoning; Animals; Bivalvia; Chromatography, Liquid; Food Contamination; Marine Toxins; Mice; Oxidation-Reduction; Shellfish Poisoning
AbstractSixteen laboratories participated in a collaborative study to evaluate method performance parameters of a liquid chromatographic method of analysis for paralytic shellfish toxins (PST) in blue mussels (Mytilus edulis), soft shell clams (Mya arenaria), sea scallops (Placopectin magellanicus), and American oysters (Crassostrea virginicus). The specific analogs tested included saxitoxin, neosaxitoxin, gonyautoxins-1 to -5, decarbamoyl-gonyautoxins-2 and -3, decarbamoyl-saxitoxin, and N-sulfocarbamoylgonyautoxin- 2 and -3. This instrumental technique has been developed as a replacement for the current AOAC biological method (AOAC Official MethodSM 959.08) and an alternative to the pre-column oxidation LC method (AOAC Official MethodSM 2005.06). The method is based on reversed-phase liquid chromatography with post-column oxidation and fluorescence detection (excitation 330 nm and emission 390 nm). The shellfish samples used in the study were prepared from the edible tissues of clams, mussels, oysters, and scallops to contain concentrations of PST representative of low, medium, and high toxicities and with varying profiles of individual toxins. These concentrations are approximately equivalent to = maximum level (ML), ML, or 2×ML established by regulatory authorities (0.40, 0.80, and 1.60 mg STẊdiHCl eq/kg, respectively). Recovery for the individual toxins ranged from 104 to 127%, and recovery of total toxin averaged 116%. Horwitz Ratio (HorRat) values for individual toxins in the materials included in the study were generally within the desired range of 0.3 to 2.0. For the estimation of total toxicity in the test materials, the reproducibility relative standard deviation ranged from 4.6 to 20%. A bridging study comparing the results from the study participants using the post-column oxidation (PCOX) method with the results obtained in the study director's laboratory on the same test materials using the accepted reference method, the mouse bioassay (MBA; AOAC Official MethodSM 959.08), showed that the average ratio of results obtained from the two methods was 1.0. A good match of values was also achieved with a new certified reference material. The results from this study demonstrated that the PCOX method is a suitable method of analysis for PST in shellfish tissue and provides both an estimate of total toxicity, equivalent to that determined using the MBA AOAC Official MethodSM 959.08, and a detailed profile of the individual toxin present in the sample. © 2012 Publishing Technology.
Publication date
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21271716
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Record identifierb790651b-e655-4d1d-8e18-84e46c72311d
Record created2014-03-24
Record modified2016-05-09
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