Multivalent display of single-domain antibodies

  1. Get@NRC: Multivalent display of single-domain antibodies (Opens in a new window)
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Journal titleMethods in Molecular Biology
Pages445456; # of pages: 12
Subjectcarcinoembryonic antigen related cell adhesion molecule 1; nanobody; pentabody ES1; plasmid vector; unclassified drug; nanobody; antigen binding; antigen recognition; article; carcinoma cell; controlled study; drug purification; drug synthesis; gene expression; human; human cell; nucleotide sequence; phage display; priority journal; amino acid sequence; animal; Artiodactyla; cell surface display; chemistry; gene expression; gene library; gene order; genetics; immunoglobulin variable region; immunology; methodology; molecular genetics; protein binding; tumor cell line; Amino Acid Sequence; Animals; Camelids, New World; Cell Line, Tumor; Cell Surface Display Techniques; Gene Expression; Gene Library; Gene Order; Humans; Immunoglobulin Variable Region; Molecular Sequence Data; Protein Binding; Single-Domain Antibodies
AbstractAntigen-binding fragments, such as single-domain antibodies (sdAbs), can now be readily isolated by in vitro technologies. Antibody fragment libraries derived from immune or nonimmune sources are presented in a molecular display format, typically phage display, and binders to individual antigens are selected from the libraries by a so-called panning process. Nonimmune libraries can serve as sources of binders to a wide range of targets but yield antigen-binding fragments that generally have much lower affinities than those obtained from immune sources. Here we describe a strategy for constructing pentameric sdAbs termed pentabodies. Pentamerization introduces avidity which can greatly enhance the binding of low affinity sdAbs to antigens presented on surfaces. © 2012 Springer Science+Business Media, LLC.
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AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21269170
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Record identifierb82ea504-ebdc-41a0-82c8-969f6e608e89
Record created2013-12-12
Record modified2016-05-09
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