Nuclear localization of prostaglandin E2 receptors

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DOIResolve DOI: http://doi.org/10.1073/pnas.95.26.15792
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TypeArticle
Journal titleProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue26
Pages1579215797; # of pages: 6
AbstractProstaglandin E2 receptors (EP) were detected by radioligand binding in nuclear fractions isolated from porcine brain and myometrium. Intracellular localization by immunocytofluorescence revealed perinuclear localization of EPs in porcine cerebral microvascular endothelial cells. Nuclear association of EP1 was also found in fibroblast Swiss 3T3 cells stably overexpressing EP1 and in human embryonic kidney 293 (Epstein舑Barr virus-encoded nuclear antigen) cells expressing EP1 fused to green fluorescent protein. High-resolution immunostaining of EP1 revealed their presence in the nuclear envelope of isolated (cultured) endothelial cells and in situ in brain (cortex) endothelial cells and neurons. Stimulation of these nuclear receptors modulate nuclear calcium and gene transcription.
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AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NPARC number12328483
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Record identifierb9222bc2-3ed2-40a9-b1ca-560e8daebba3
Record created2009-09-10
Record modified2016-05-09
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