Prediction of fetal lung maturity from near-infrared spectra of amniotic fluid

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DOIResolve DOI: http://doi.org/10.1016/S0020-7292(97)02892-0
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TypeArticle
Journal titleInternational Journal of Gynecology & Obstetrics
ISSN0020-7292
Volume57
Issue2
Pages161168; # of pages: 8
SubjectNear-infrared spectra; Phospholipid profile; Amniocentesis; Fetal lung maturity
AbstractObjective: The aim of this study was to attempt to correlate the quantitative data obtained from the near-infrared (near-IR) spectra of amniotic fluid with the lecithin/sphingomyelin (LS) ratio determined from thin layer chromatographic analysis on the same samples, in order to establish the feasibility of near-IR spectroscopy as an alternate method for the prediction of fetal lung development. Methods: Samples of amniotic fluid were obtained by amniocentesis from 52 patients between the 26th and 41st week of pregnancy. About 350 μl of amniotic fluid was required to record the near-IR spectrum over the entire spectral range between 400 and 2500 nm using a commercial spectrometer. The LS ratio was determined independently by thin layer chromatography (TLC) for each sample. For correlating the infrared spectra with the TLC-based LS ratios, a partial least squares analysis was used. Results: The LS ratios predicted from the near-IR spectra of amniotic fluid were highly correlated and in excellent agreement with those determined by TLC (r = 0.91). Conclusion: Near-IR spectroscopy has the potential to become an alternate method to TLC for prediction of fetal pulmonary maturity.
Publication date
PublisherElsevier B.V.
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NRC number499
NPARC number9148314
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Record identifierb976e87e-ddd2-47c4-9f10-a9ff9bf42517
Record created2009-06-25
Record modified2016-10-31
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