At-line monitoring of bioreactor protein production by surface plasmon resonance

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DOIResolve DOI: http://doi.org/10.1002/bit.21725
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TypeArticle
Journal titleBiotechnology and Bioengineering
Volume100
IssueMay 1
Pages184188; # of pages: 5
Subjectat-line protein monitoring; bioreactor protein production; HEK 293 cells; surface plasmon resonance; transient transfection
AbstractAn innovative and automated method for the at-line monitoring of secreted protein was developed by harnessing a Surface Plasmon Resonance-based biosensor to a bioreactor. The proof of concept was performed by following at-line the relative concentration of a secreted protein produced by transient transfection of mammalian cells in a bioreactor. Our results suggest that our approach can be readily applied to the at-line determination of both protein concentration and bioactivity. Our experimental setup and strategy can thus satisfy the needs related to the development of novel bioprocess control protocols in the context of the new process analytical technology that arises in the biopharmaceutical industry.
Publication date
PublisherWiley
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Access conditionavailable
unlimited
public
NoteDA - 20080402IS - 1097-0290 (Electronic)LA - engPT - Journal ArticlePT - Research Support, Non-U.S. Gov'tRN - 0 (Recombinant Proteins)SB - IM
Peer reviewedYes
NRC number47823
47823
NPARC number12400992
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Record identifierb99769d5-7e41-4be6-af86-fc06a4292628
Record created2009-10-02
Record modified2016-05-09
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