A sensitive electrochemical assay for early detection of HIV-1 protease using ferrocene-peptide conjugate/au nanoparticle/single walled carbon nanotube modified electrode

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DOIResolve DOI: http://doi.org/10.1080/00032711003653924
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TypeArticle
Journal titleAnalytical letters
Volume43
Issue10
Pages16801687; # of pages: 8
SubjectElectrochemistry; Ferrocene; Gold nanoparticle; HIV-1; Pepstatin; Protease; Single-walled carbon nanotube
AbstractA rapid and simple electrochemical detection method has been developed to detect the presence of protease (HIV-1 PR) in HIV patients' sera. In this assay format, disposable screen printed gold electrode (SPGE) surface was modified with gold nanoparticles (AuNPs) or thiolated single-walled carbon nanotubes/gold nanoparticles (SWCNT/AuNPs). Thiol-terminated ferrocene (Fc)-pepstatin was then self-assembled on such modified surfaces. The interaction between the Fc-pepstatin modified substrates and HIV-1 PR was confirmed by scanning electron (SEM) and atomic force microscopy (AFM) and probed by differential pulse voltammetry (DPV). A linear relationship was observed between the shift in peak potential and the increasing number of HIV-1 viral replications in the HIV-infected serum samples.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute
Peer reviewedYes
NRC number53331
NPARC number16152640
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Record identifierba6993f8-34a6-4794-9c29-b41273f26f22
Record created2010-11-05
Record modified2016-05-09
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