Development of a SYBR safe TM technique for the sensitive detection of DNA in cesium chloride density gradients for stable isotope probing assays

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DOIResolve DOI: http://doi.org/10.1016/j.mimet.2008.01.016
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TypeArticle
Journal titleJournal of Microbiological Methods
Volume73
Issue2
Pages199202; # of pages: 4
SubjectENV; stable isotope probing; cesium chloride density gradient; SYBR safe™; ethidium bromide
AbstractSYBR safe™, a fluorescent nucleic acid stain, was evaluated as a replacement for ethidium bromide (EtBr) in cesium chloride (CsCl) density gradients for DNA stable isotope probing (DNA-SIP) assays. The separation of ¹²C- and ¹³C-labelled DNA using SYBR safe™ gave similar results to those obtained using EtBr with pure cultures and environmental samples exposed to a ¹³C-labelled substrate, while the detection limit of DNA was enhanced by the use of SYBR safe™ by at least 5 times. The results demonstrated that SYBR safe™ is a safe, sensitive and effective alternative to the use of ethidium bromide in CsCl density gradients for DNA-SIP assays.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number49950
NPARC number12465408
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Record identifierbadd1c5b-cc10-478e-9c09-46cc5f6de442
Record created2009-10-26
Record modified2016-05-09
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