Utilization of Fluorescent Microspheres and a Green Fluorescent Protein-Marked Strain for Assessment of Microbiological Contamination of Permafrost and Ground Ice Core Samples from the Canadian High Arctic

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DOIResolve DOI: http://doi.org/10.1128/AEM.71.2.1035-1041.2005
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TypeArticle
Journal titleAcoustics, Speech, and Signal Processing Newsletter, IEEE
Volume71
Issue2
Pages10351041; # of pages: 7
AbstractFluorescent microspheres were applied in a novel fashion during subsurface drilling of permafrost and ground ice in the Canadian High Arctic to monitor the exogenous microbiological contamination of core samples obtained during the drilling process. Prior to each drill run, a concentrated fluorescent microsphere (0.5-{micro}m diameter) solution was applied to the interior surfaces of the drill bit, core catcher, and core tube and allowed to dry. Macroscopic examination in the field demonstrated reliable transfer of the microspheres to core samples, while detailed microscopic examination revealed penetration levels of less than 1 cm from the core exterior. To monitor for microbial contamination during downstream processing of the permafrost and ground ice cores, a Pseudomonas strain expressing the green fluorescent protein (GFP) was painted on the core exterior prior to processing. Contamination of the processed core interiors with the GFP-expressing strain was not detected by culturing the samples or by PCR to detect the gfp marker gene. These methodologies were quick, were easy to apply, and should help to monitor the exogenous microbiological contamination of pristine permafrost and ground ice samples for downstream culture-dependent and culture-independent microbial analyses.
Publication date
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute; NRC Steacie Institute for Molecular Sciences
Peer reviewedNo
NPARC number12332839
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Record identifierbb14390d-f2b1-44dd-baf8-0127785080a7
Record created2009-09-10
Record modified2016-05-09
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