Label-free imaging of arterial tissues using photonic crystal fibre (PCF) based nonlinear optical microscopic system

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Proceedings titleProceedings of SPIE
ConferenceImaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 26 January 2009, San Jose, CA, USA
SubjectNonlinear optical microscopy; photonic crystal fibre; coherent anti-Stokes Raman scattering; two photon excited fluorescence; second harmonic generation; Atherosclerosis
AbstractNonlinear optical (NLO) microscopy provides a minimally invasive optical method for fast molecular imaging at subcellular resolution with 3D sectioning capability in thick, highly scattering biological tissues. In the current study, we demonstrate the imaging of arterial tissue using a nonlinear optical microscope based on photonic crystal fiber and a single femto-second oscillator operating at 800nm. This NLO microscope system is capable of simultaneous imaging extracellular elastin/collagen structures and lipid distribution within aortic tissue obtained from coronary atherosclerosis-prone WHHLMI rabbits (Watanabe heritable hyperlipidemic rabbit-myocardial infarction) Clear pathological differences in arterial lumen surface were observed between healthy arterial tissue and atherosclerotic lesions through NLO imaging.
Publication date
AffiliationNRC Institute for Biodiagnostics; NRC Steacie Institute for Molecular Sciences; National Research Council Canada
Peer reviewedYes
NPARC number19697993
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Record identifierbb462be8-6197-47c9-af3e-09cc07c8a5b3
Record created2012-03-22
Record modified2016-05-09
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