A mussel tissue certified reference material for multiple phycotoxins. Part 1: design and preparation

Download
  1. Get@NRC: A mussel tissue certified reference material for multiple phycotoxins. Part 1: design and preparation (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1007/s00216-011-4786-9
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleAnalytical and Bioanalytical Chemistry
Volume400
Issue3
Pages821833; # of pages: 13
SubjectAccuracy; Certified reference material; CRM-FDMT1; Liquid chromatography-mass spectrometry; Phycotoxins; Precision; Shellfish toxins
AbstractThe development of multi-analyte methods for lipophilic shellfish toxins based on liquid chromatography-mass spectrometry permits rapid screening and analysis of samples for a wide variety of toxins in a single run. Validated methods and appropriate certified reference materials (CRMs) are required to ensure accuracy of results. CRMs are essential for accurate instrument calibration, for assessing the complete analytical method from sample extraction to data analysis and for verifying trueness. However, CRMs have hitherto only been available for single toxin groups. Production of a CRM containing six major toxin groups was achieved through an international collaboration. Preparation of this material, CRM-FDMT1, drew on information from earlier studies as well as improved methods for isolation of toxins, handling bulk tissues and production of reference materials. Previous investigations of stabilisation techniques indicated freeze-drying to be a suitable procedure for preparation of shellfish toxin reference materials and applicable to a wide range of toxins. CRM-FDMT1 was initially prepared as a bulk wet tissue homogenate containing domoic acid, okadaic acid, dinophysistoxins, azaspiracids, pectenotoxin-2, yessotoxin and 13-desmethylspirolide C. The homogenate was then freeze-dried, milled and bottled in aliquots suitable for distribution and analysis. The moisture content and particle size distribution were measured, and determined to be appropriate. A preliminary toxin analysis of the final material showed a comprehensive toxin profile. [Figure not available: see fulltext.] © 2011 Her Majesty the Queen in Right of Canada.
Publication date
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NPARC number19770589
Export citationExport as RIS
Report a correctionReport a correction
Record identifierbb7a505d-984d-452a-8793-4f12ab2dc0c6
Record created2012-04-04
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)