Identification of Hitherto Undefined B-Cell Epitopes by Antibodies in the Sera of Vitiligo Patients Using Phage-Display Peptide Library: Iran J.Allergy Asthma Immunol.

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TypeArticle
Journal titleIran J.Allergy Asthma Immunol.
Volume2
Issue4
Pages197201; # of pages: 5
SubjectACID; amino acid; Amino Acid Sequence; AMINO-ACID; AMINO-ACID-SEQUENCE; Antibodies; antibody; epitope; Epitopes; IDENTIFICATION; immunology; Libraries; peptide; Peptide Library; Peptides; SEQUENCE; SEQUENCES; Serum
AbstractA random 12 mers phage library was used to screen a pool of immunoglobulin fractions obtained from vitiligo patients. Subsequent to panning experiments, a panel of affinity selected phage from vitiligo patients were obtained. This panel was tested using an ELISA for their reactivity with pooled sera from patients and normal controls. Among the 16 randomly selected clones, two of clones showed distinct positive reactivity with the patient's sera compared with controls. The peptides displayed by these phages expressed the following amino acid sequences: SHMPLANQYQWA and NHVQAWEQFWDS. Thus, screening with phagedisplayed random peptide library of vitiligo sera can reveal peptide sequences that mimic vitiligo-related self-antigen
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NRC numberJADALI1Z2003
NPARC number9386336
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Record identifierbbc46077-5b83-4f97-aa3f-452c9483d720
Record created2009-07-10
Record modified2016-05-09
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