Single-Domain Antibody Bioconjugated Near-IR Quantum Dots for Targeted Cellular Imaging of Pancreatic Cancer

Download
  1. (PDF, 718 KB)
  2. Get@NRC: Single-Domain Antibody Bioconjugated Near-IR Quantum Dots for Targeted Cellular Imaging of Pancreatic Cancer (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1166/jnn.2011.4167
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleJournal of Nanoscience and Nanotechnology
Volume11
Issue5
Pages37573763; # of pages: 7
SubjectPhotoluminescent Near-IR Quantum Dots; Single-Domain Antibody; In Vitro Targeted Cellular Imaging; Colloidal Nanocrystals; Pancreatic and Breast Cancer
AbstractSuccessful targeted imaging of BxPC3 human pancreatic cancer cells is feasible with near-IR CdTeSe/CdS quantum dots (QDs) functionalized with single-domain antibody (sdAb) 2A3. For specific targeting, sdAbs are superior to conventional antibodies, especially in terms of stability, aggregation, and production cost. The bright CdTeSe/CdS QDs were synthesized to emit in the diagnostic window of 650-900 nm with a narrow emission band. 2A3 was derived from llama and is small in size of ∼13 kDa, but with fully-functional recognition to the target carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), a possible biomarker as a therapeutic target of pancreatic cancer. For compelling imaging, optical may be the most sensible among the various imaging modalities, regarding the sensitivity and cost. This first report on sdAb-conjugated near-IR QDs with high signal to background sensitivity for targeted cellular imaging brings insights into the development of optical molecular imaging for early stage cancer diagnosis.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Institute for Biological Sciences; NRC Steacie Institute for Molecular Sciences; NRC Institute for Microstructural Sciences
Peer reviewedNo
NPARC number17652983
Export citationExport as RIS
Report a correctionReport a correction
Record identifierbc8a1ff6-0b8b-4268-a243-69ff2cef5943
Record created2011-03-31
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)