Identification and characterization of MFA1, the gene encoding Candida albicans a-factor pheromone

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DOIResolve DOI: http://doi.org/10.1128/EC.00387-06
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TypeArticle
Journal titleEukaryotic Cell
Volume6
Issue3
Pages487494; # of pages: 8
SubjectAcids; Amino Acids; analysis; Biotechnology; Candida; Candida albicans; Cells; Genes; genome; Genome sequence; pha; Protein; Proteins; Saccharomyces cerevisiae
AbstractIn the opaque state, MTLa and MTLalpha strains of Candida albicans are able to mate, and this mating is directed by a pheromone-mediated signaling process. We have used comparisons of genome sequences to identify a C. albicans gene encoding a candidate a-specific mating factor. This gene is conserved in Candida dubliniensis and is similar to a three-gene family in the related fungus Candida parapsilosis but has extremely limited similarity to the Saccharomyces cerevisiae MFA1 (ScMFA1) and ScMFA2 genes. All these genes encode C-terminal CAAX box motifs characteristic of prenylated proteins. The C. albicans gene, designated CaMFA1, is found on chromosome 2 between ORF19.2165 and ORF19.2219. MFA1 encodes an open reading frame of 42 amino acids that is predicted to be processed to a 14-amino-acid prenylated mature pheromone. Microarray analysis shows that MFA1 is poorly expressed in opaque MTLa cells but is induced when the cells are treated with alpha-factor. Disruption of this C. albicans gene blocks the mating of MTLa cells but not MTLalpha cells, while the reintegration of the gene suppresses this cell-type-specific mating defect
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number47551
NPARC number3540322
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Record identifierbc9aa966-150c-4113-8891-8fbd8499068a
Record created2009-03-01
Record modified2016-05-09
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