Validation of a single-isotope-labeled cholesterol tracer approach for measuring human cholesterol absorption

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DOIResolve DOI: http://doi.org/10.1007/s11745-004-1206-6
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TypeArticle
Journal titleLipids
ISSN0024-4201
1558-9307
Volume39
IssueJanuary 1
Pages8791; # of pages: 5
SubjectAged; cross-over studies; fatty acids; female; humans; ipoproteins, LDL/blood; Lipoproteins, HDL/blood; triglycerides
AbstractCholesterol absorption is frequently determined using the plasma dual stable-isotope ratio method (PDSIRM). However, this method involves intravenous injection of stableisotope-labeled cholesterol with simultaneous oral administration of differently labeled cholesterol, which results in high study costs and involves additional ethical considerations. The objective of the present study was to validate a simpler singleisotope method for determining cholesterol absorption against PDSIRM by using data from two previous studies. Enrichments of carbon-13 (¹³C) and deuterium in red blood cells were analyzed by using differential isotope ratio MS. The area under the curve of ¹³C-enrichment in the plasma free-cholesterol pool was found to be significantly correlated with cholesterol absorption measured by using PDSIRM for study 1 (r = 0.85, P < 0.0001) and study 2 (r = 0.81, P < 0.0001). Average ¹³C-enrichment correlated with the area under the curve of ¹³C-enrichment in the plasma free cholesterol for both study 1 (r = 0.98, P < 0.0001) and study 2 (r = 1.00, P < 0.0001). Study 1 examined the efficacy and mechanisms of unesterified plant sterols and stanols on lipid profiles in hypercholesterolemic men and women, while study 2 investigated the effects of phytosterol vs. phytostanol esters on plasma lipid levels and cholesterol kinetics in hyperlipidemic men. Experimental approaches to determine cholesterol absorption were identical between the two studies. Consequently, in both studies, correlations (r = 0.88, P < 0.0001 for study 1, and r = 0.82, P < 0.0001 for study 2) were found between the average ¹³C-enrichment of plasma free cholesterol and cholesterol absorption measured by PDSIRM. These results suggest that a single-isotope-labeled cholesterol tracer approach can be used as a reliable noninvasive method to replace PDSIRM for examining changes in cholesterol absorption.
Publication date
PublisherSpringer
Copyright noticeThe material in this document is covered by the provisions of the Copyright Act, by Canadian laws, policies, regulations and international agreements. Such provisions serve to identify the information source and, in specific instances, to prohibit reproduction of materials without written permission
LanguageEnglish
AffiliationNRC Institute for Nutrisciences and Health (INH-ISNS); National Research Council Canada
Access conditionavailable
unlimited
public
Peer reviewedYes
NRC publication
This is a non-NRC publication

"Non-NRC publications" are publications authored by NRC employees prior to their employment by NRC.

NRC number54696
NPARC number9148845
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Record identifierbd6d81fb-e1e7-4ffe-86d4-d7492a948038
Record created2009-10-02
Record modified2016-05-09
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