Structural characterization of the extracellular polysaccharide from Vibrio cholerae O1 El-Tor

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DOIResolve DOI: http://doi.org/10.1371/journal.pone.0086751
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TypeArticle
Journal titlePLoS ONE
ISSN1932-6203
Volume9
Issue1
Article numbere86751
Subjectexopolysaccharide; glucosamine; glycine; glycoconjugate; hydrochloric acid; n acetylglucosamine; biofilm; carbohydrate analysis; chemical binding; chemical modification; extracellular matrix; isolation procedure; proton nuclear magnetic resonance; structure activity relation; structure analysis; Vibrio cholerae; viscosity
AbstractThe ability to form biofilms is important for environmental survival, transmission, and infectivity of Vibrio cholerae, the causative agent of cholera in humans. To form biofilms, V. cholerae produces an extracellular matrix composed of proteins, nucleic acids and a glycoconjugate, termed Vibrio exopolysaccharide (VPS). Here, we present the data on isolation and characterization of the polysaccharide part of the VPS (VPS-PS), which has the following structure: -4)-α-GulpNAcAGly3OAc-(1-4)-β-D-Glcp-(1-4)- α-Glcp-(1-4)-α-D-Galp-(1- where α-D-Glc is partially (∼20%) replaced with α-D-GlcNAc. α-GulNAcAGly is an amide between 2-acetamido-2-deoxy-α-guluronic acid and glycine. Apparently, the polysaccharide is bound to a yet unidentified component, which gives it high viscosity and completely suppresses any NMR signals belonging to the sugar chains of the VPS. The only reliable method to remove this component at present is a treatment of the whole glycoconjugate with concentrated hydrochloric acid.
Publication date
PublisherPLOS
LanguageEnglish
AffiliationNational Research Council Canada; Human Health Therapeutics
Peer reviewedYes
NPARC number21272635
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Record identifierbeb13b06-1d70-4430-9d37-4f2418909926
Record created2014-12-03
Record modified2016-05-09
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