Web-based inference of biological patterns, functions and pathways from metabolomic data using MetaboAnalyst

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DOIResolve DOI: http://doi.org/10.1038/nprot.2011.319
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TypeArticle
Journal titleNature Protocols
ISSN1754-2189
Volume6
Issue6
Pages743760; # of pages: 18
Subjectarticle; biology; computer program; information processing; metabolite; metabolomics; multivariate analysis; priority journal; univariate analysis; web browser; Data Interpretation, Statistical; Internet; Metabolic Networks and Pathways; Metabolomics; Models, Biological; Multivariate Analysis; Software
AbstractMetaboAnalyst is an integrated web-based platform for comprehensive analysis of quantitative metabolomic data. It is designed to be used by biologists (with little or no background in statistics) to perform a variety of complex metabolomic data analysis tasks. These include data processing, data normalization, statistical analysis and high-level functional interpretation. This protocol provides a step-wise description on how to format and upload data to MetaboAnalyst, how to process and normalize data, how to identify significant features and patterns through univariate and multivariate statistical methods and, finally, how to use metabolite set enrichment analysis and metabolic pathway analysis to help elucidate possible biological mechanisms. The complete protocol can be executed in ∼45 min. © 2011 Nature America, Inc. All rights reserved.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); National Institute for Nanotechnology (NINT-INNT)
Peer reviewedYes
NPARC number21271284
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Record identifierbf22bcec-afb3-4a14-8c1a-8d3137665d35
Record created2014-03-24
Record modified2016-05-09
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