Lepeophtheirus salmonis secretory/excetory products and their effects on Atlantic salmon immune gene expression

Alternative titleParasite Immunology
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DOIResolve DOI: http://doi.org/10.1111/j.1365-3024.2007.00932.x
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TypeArticle
Journal titleParasite Immunology
Volume29
Issue4
Pages179189; # of pages: 11
Subjectgene expression; immunomodulation; Salmo salar sea lice
AbstractSummary: We have previously shown that Lepeophtheirus salmonis produces trypsin and prostaglandin E2 (PGE2) that are most likely responsible for the limited inflammatory response of Atlantic salmon to infection. After removal of the dopamine and PGE2, the immunomodulatory activity of unfractionated and pools of the fractionated secretions was determined by examining the effects of the secretions on Atlantic salmon immune gene expression. Incubation of macrophage-enriched isolates of Atlantic salmon head kidney cells with the unfractionated secretion + PGE2 revealed a significant inhibition of interleukin-1? (IL-1?) and major histocompatibility class I gene expression. Inhibition of lipopolysaccharide-induced IL-1? expression in the Atlantic salmon head kidney cell line (SHK-1) was observed when three pools of the secretory/excretory products were tested. Further purification of products within these pools revealed that fraction 1-2 could account fully for the inhibition of IL-1? expression in SHK-1 cells observed in pooled fraction 1. This study demonstrates that there are other immunomodulatory compounds produced by L. salmonis, in addition to PGE2 and trypsin, that can inhibit the expression of Atlantic salmon immune-related genes in vitro.
Publication date
Copyright noticeCopyright © 2007, Blackwell Publishing Ltd.
LanguageEnglish
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada; NRC Genomics and Health Initiative; Human Health Therapeutics; Industrial Research Assistance Program
Peer reviewedYes
NRC number42643
1232
NPARC number3538427
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Record identifierbfea9e4d-5557-41cf-8620-9093b4e48d26
Record created2009-03-01
Record modified2016-05-09
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