Plasticity in cell division patterns and auxin transport dependency during in vitro embryogenesis in Brassica napus

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DOIResolve DOI: http://doi.org/10.1105/tpc.114.126300
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TypeArticle
Journal titlePlant Cell
ISSN1040-4651
Volume26
Issue6
Pages25682581; # of pages: 14
AbstractIn Arabidopsis thaliana, zygotic embryo divisions are highly regular, but it is not clear how embryo patterning is established in species or culture systems with irregular cell divisions. We investigated this using the Brassica napus microspore embryogenesis system, where the male gametophyte is reprogrammed in vitro to form haploid embryos in the absence of exogenous growth regulators. Microspore embryos are formed via two pathways: a zygotic-like pathway, characterized by initial suspensor formation followed by embryo proper formation from the distal cell of the suspensor, and a pathway characterized by initially unorganized embryos lacking a suspensor. Using embryo fate and auxin markers, we show that the zygotic-like pathway requires polar auxin transport for embryo proper specification from the suspensor, while the suspensorless pathway is polar auxin transport independent and marked by an initial auxin maximum, suggesting early embryo proper establishment in the absence of a basal suspensor. Polarity establishment in this suspensorless pathway was triggered and guided by rupture of the pollen exine. Irregular division patterns did not affect cell fate establishment in either pathway. These results confirm the importance of the suspensor and suspensor-driven auxin transport in patterning, but also uncover a mechanism where cell patterning is less regular and independent of auxin transport.
Publication date
PublisherAmerican Society of Plant Biologists
LanguageEnglish
AffiliationNational Research Council Canada; Aquatic and Crop Resource Development
Peer reviewedYes
NRC numberNRC-ACRD-56075
NPARC number21272697
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Record identifierc02173fb-e819-4601-9384-4b6303eaeeca
Record created2014-12-03
Record modified2016-05-09
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