A combined vibrational sum frequency generation spectroscopy and atomic force microscopy study of sphingomyelin–cholesterol monolayers

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DOIResolve DOI: http://doi.org/10.1021/la301332e
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TypeArticle
Journal titleLangmuir
ISSN0743-7463
1520-5827
Volume28
Issue36
Pages1299913007
AbstractA combination of vibrational sum frequency generation spectroscopy and atomic force microscopy is used to study the changes in morphology and conformational order in monolayers prepared from three natural sphingomyelin (SM) mixtures as a function of surface pressure and cholesterol concentration. The most homogeneous SM gave monolayers with well-ordered acyl chains and few gauche defects with relatively small effects of either increasing surface pressure or cholesterol addition. Heterogeneous SM mixtures with a mixture of acyl chain lengths or with significant fractions of unsaturated acyl chains had much larger contributions from gauche defects at low surface pressure and gave increasingly well-ordered monolayers as the surface pressure increased. They also showed substantial increases in lipid chain order after cholesterol addition. Overall, these results are consistent with the strong hydrogen bonding capacity of SM leading to well-ordered monolayers over a range of surface pressures. The changes in acyl chain order for natural SMs as a function of cholesterol are relevant to formation of sphingolipid–cholesterol enriched domains in cell membranes.
Publication date
LanguageEnglish
AffiliationSecurity and Disruptive Technologies; National Research Council Canada
Peer reviewedYes
NPARC number21268902
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Record identifierc0cf24dd-44f5-4cb9-834e-08a1727b427b
Record created2013-11-25
Record modified2016-05-09
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