Analysis of insecticidal proteins from Bacillus thuringiensis and recombinant Escherichia coli by capillary electrokinetic chromatography

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DOIResolve DOI: http://doi.org/10.1002/elps.200406046
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TypeArticle
Journal titleElectrophoresis
Volume25
Issue18-19
Pages32923299; # of pages: 8
Subjectanalysis; env; Escherichia coli; Proteins; Protein
AbstractBacillus thuringiensis and recombinant Escherichia coli proteinaceous protoxins were subject to proteolysis and analyzed by capillary electrokinetic chromatography. Three resulting toxins (65 kDa) were baseline-resolved within 22 min using a 10 mM borate, pH 11 separation buffer consisting of 25 mM sodium dodecyl sulfate (SDS) and 30 mm phytic acid. The toxins displayed differential interactions with the SDS and phytic acid phases to effect their separation. The ion-pairing interaction between the analyte and phytic acid was also useful in preventing adsorption to the capillary walls and thus enhanced separation resolution and efficiency. The use of electrokinetic chromatography allows achievement of the separation in a significantly shorter time than conventional high-performance liquid chromatography (HPLC) using a diethylaminoethyl (DEAE) weak-anion exchanger.
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number47192
NPARC number3538718
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Record identifierc105fd18-d4b0-4cf7-a15d-74a184964616
Record created2009-03-01
Record modified2016-05-09
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