Portable bead-based fluorescence detection system for multiplex nucleic acid testing: a case study with Bacillus anthracis

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DOIResolve DOI: http://doi.org/10.1007/s10404-013-1273-y
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TypeArticle
Journal titleMicrofluidics and Nanofluidics
ISSN1613-4982
Pages113; # of pages: 13
AbstractThis paper describes the design, functioning and use of a portable detection platform for multiplex nucleic acid testing. The system features a bead-supported DNA hybridization assay performed inside a microfluidic cartridge. Polystyrene particles modified with DNA capture probes are confined in the detection area and exposed to a solution of fluorescently labeled target DNA strands. The cartridge, fabricated from inexpensive thermoplastic polymers, allows for conducting up to eight assays in parallel. The detection instrument is equipped with a pneumatic module and a manifold lid serving as an interface to mediate fluid displacement on the cartridge. The fluorescence signal deriving from each assay is recorded by a semi-confocal fluorescence reader embedded in the detection platform. The compact design of the instrument and its level of integration make it possible to obtain an analytical result in less than 15 min, while only few manual steps need to be performed in between. A proof-of-concept demonstration involving Cy3-labeled, PCR-amplified genomic DNA confirms the ability to detect Bacillus anthracis in a multiplexed single-assay format using lef and capC genes. Limits of quantification are on the order of 1 × 109 copies/μL for lef targets. © 2013 Springer-Verlag Berlin Heidelberg.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); Medical Devices
Peer reviewedYes
NPARC number21271823
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Record identifierc1d3c7ab-2567-4eaf-b21d-e1d6c98416fb
Record created2014-04-22
Record modified2016-05-09
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