Association of anti-GT1a antibodies with an outbreak of Guillain-Barré syndrome and analysis of ganglioside mimicry in an associated Campylobacter jejuni strain

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DOIResolve DOI: http://doi.org/10.1371/journal.pone.0131730
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TypeArticle
Journal titlePLoS ONE
ISSN1932-6203
Volume10
Issue7
Article numbere0131730
Subjectanti-ganglioside antibody; phylogenetic analysis; antigenic drift; ganglioside antibody; ganglioside GD 1b; ganglioside GD3; ganglioside GM1; ganglioside GM2; ganglioside GQ 1b; ganglioside GT 1a; glycosyltransferase; immunoglobulin G antibody; immunoglobulin M antibody; lipooligosaccharide; antibody blood level; bacterial genome; bacterial strain; cgtA gene; cgtB gene; cst II gene; diarrhea; DNA sequence; gastroenteritis; molecular mimicry; phylogenetic tree; phylogeny; single nucleotide polymorphism
AbstractAn outbreak of Guillain-Barré syndrome (GBS), subsequent to Campylobacter jejuni enteritis, occurred in China in 2007. Serum anti-ganglioside antibodies were measured in GBS patients and controls. Genome sequencing was used to determine the phylogenetic relationship among three C. jejuni strains from a patient with GBS (ICDCCJ07001), a patient with gastroenteritis (ICDCCJ07002) and a healthy carrier (ICDCCJ07004), which were all associated with the outbreak. The ganglioside-like structures of the lipo-oligosaccharides of these strains were determined by mass spectrometry. Seventeen (53%) of the GBS patients had anti-GT1a IgG antibodies. GT1a mimicry was found in the lipo-oligosaccharides of strain ICDCCJ07002 and ICDCCJ07004; but a combination of GM3/GD3 mimics was observed in ICDCCJ07001, although this patient had anti-GT1a IgG antibodies. A single-base deletion in a glycosyltransferase gene caused the absence of GT1a mimicry in ICDCCJ07001. The phylogenetic tree showed that ICDCCJ07002 and ICDCCJ07004 were genetically closer to each other than to ICDCCJ07001. C. jejuni, bearing a GT1a-like lipo-oligosaccharide, might have caused the GBS outbreak and the loss of GT1a mimicry may have helped ICDCCJ07001 to survive in the host.
Publication date
PublisherPLOS
LanguageEnglish
AffiliationNational Research Council Canada; Human Health Therapeutics
Peer reviewedYes
NRC numberNRC-HHT-53231
NPARC number21275343
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Record identifierc29b6749-3a3c-4e6d-85b8-81bc3b0df95c
Record created2015-06-15
Record modified2016-05-09
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