Microbial contamination of embryos and semen during long term banking in liquid nitrogen

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DOIResolve DOI: http://doi.org/10.1016/S0011-2240(03)00020-8
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TypeArticle
Journal titleCryobiology
Volume46
Issue2
Pages146152; # of pages: 7
Subjectenv; Nitrogen; Bacteria; Escherichia coli; Humans; Human; Animals; Animal; In Vitro; analysis; Electrophoresis
AbstractWe report on microbial contamination of embryos and semen cryopreserved in sealed plastic straws and stored for 6-35 years in liquid nitrogen. There were 32 bacterial and 1 fungal species identified from randomly drawn liquid nitrogen, frozen semen, and embryos samples stored in 8 commercial and 8 research facility liquid nitrogen (LN) tanks. The identified bacteria represented commensal or environmental microorganisms and some, such as Escherichia coli, were potential or opportunistic pathogens for humans and animals. Stenotrophomonas maltophilia was the most common contaminant identified from the samples and was further shown to significantly suppress fertilization and embryonic development in vitro. Analysis of the strains by pulsed field gel electrophoresis revealed restriction patterns with no relatedness indicating that there was no apparent cross-contamination of S. maltophilia strains between the germplasm and liquid nitrogen samples. In addition, no transmission of bovine viral diarrhea virus (BVDV) and bovine herpesvirus-1 (BHV-1) from infected semen and embryos straws to clean germplasm stored in the same LN tanks or LN was detected. (c) Biosciences Information Services.
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number45900
NPARC number3538722
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Record identifierc3616796-f134-443c-9bc3-6931250e8bc0
Record created2009-03-01
Record modified2016-05-09
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