Flow cytometric determination of cell cycles and growth rates of Prorocentrum SPP

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EditorSearch for: Reguera, B.; Search for: Blanco, J.; Search for: Fernandez, M. L.; Search for: Wyatt, T.
Proceedings titleProceedings of the VIII International Conference on Harmful Algae
ConferenceEighth International Conference on Harmful Algae, 25-29 June 1997, Vigo, Spain
Pages173176; # of pages: 4
AbstractCell cycles and growth rates of the bloom-forming marine dinoflagellates Prorocentrum micans and P. minimum were studied in laboratory batch cultures. Flow cytometry was used for analysis of the cell cycle over a 96 h period. In both species, nuclear DNA synthesis (S phase) was discrete. The duration of S phase in P. micans (14 h) was significantly longer than that in P. minimum (6-8 h), which was attributable to the larger amount of nuclear DNA in P. micans cells. Cell cycle phases (Gl, S, G2+M) were linked to the photoperiod. Cytokinesis always occurred during the first half of the light period. Growth rates derived from cell cycle analysis were always higher than those calculated from cell numbers and chlorophyll a. This discrepancy may be due to death of cells and/or incomplete duplication of chlorophyll during successive cycles of cell division. Chlorophyll synthesis was phased to the cell cycle but there were significant differences between species. In P. micans, chlorophyll synthesis usually started before cytokinesis (during late S phase), 3-5 h before the dark to light transition, and continued as long as 10 h. In contrast, in P. minimum, chlorophyll synthesis occurred only after cytokinesis, within a narrow window of 4-6 h during early Gl phase in the light. Although chlorophyll synthesis among Prorocentrum species is coupled to their ..respective cell cycle phases, no universal circadian pattern exists for photoautotrophic dinoflagellates.
Publication date
PublisherXunta de Galicia and Intergovernmental Oceanographic Commission of UNESCO
AffiliationNRC Institute for Marine Biosciences; National Research Council Canada
Peer reviewedYes
NPARC number21264662
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Record identifierc3f90b25-9bcf-442a-873e-d6afc8137db2
Record created2013-03-20
Record modified2016-05-09
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