Transient gene expression in serum-free suspension-growing mammalian cells for the production of Foot-and-mouth disease virus empty capsids

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DOIResolve DOI: http://doi.org/10.1371/journal.pone.0072800
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TypeArticle
Journal titlePLoS ONE
ISSN1932-6203
Volume8
Issue8
Article numbere72800
Subjectpolyethyleneimine; protein VP0; protein VP1; protein VP3; pTT5 vector; recombinant subunit vaccine; subunit vaccine; unclassified drug; virus protein; virus vector; animal cell; animal experiment; article; cell growth; controlled study; cost effectiveness analysis; empty virus capsid; foot and mouth disease; Foot and mouth disease virus; gene; gene cassette; genetic transfection; human; human cell; male; mammal cell; mouse; nonhuman; p12A gene; P12A3C gene; transient expression; vaccine production; virus capsid; virus immunity; VP0 gene; vp1 gene; VP3 gene
AbstractFoot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals. It produces severe economic losses in the livestock industry. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of empty capsids as a subunit vaccine has been reported to be a promising candidate because it avoids the use of virus in the vaccine production and conserves the conformational epitopes of the virus. In this report, we explored transient gene expression (TGE) in serum-free suspension-growing mammalian cells for the production of FMDV recombinant empty capsids as a subunit vaccine. The recombinant proteins produced, assembled into empty capsids and induced protective immune response against viral challenge in mice. Furthermore, they were recognized by anti-FMDV bovine sera. By using this technology, we were able to achieve expression levels that are compatible with the development of a vaccine. Thus, TGE of mammalian cells is an easy to perform, scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. © 2013 Mignaqui et al.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC); NRC Biotechnology Research Institute (BRI-IRB)
Peer reviewedYes
NPARC number21269584
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Record identifierc524b0e0-cbc3-4706-a42f-d658d07a7119
Record created2013-12-13
Record modified2016-05-09
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