Immobilization of glucose oxidase into a nanoporous TiO2 film layered on metallophthalocyanine modified vertically-aligned carbon nanotubes for efficient direct electron transfer

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DOIResolve DOI: http://doi.org/10.1016/j.bios.2013.02.029
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TypeArticle
Journal titleBiosensors and Bioelectronics
ISSN0956-5663
Volume46
Pages113118; # of pages: 6
SubjectAmperometric response; Bioelectrocatalytic; Carbon nanotubes (CNT); Detection sensitivity; Direct electron transfer; Iron phthalocyanines; Metallophthalocyanines; Nano-porous TiO; Carbon nanotubes; Glucose; Glucose oxidase; Glucose sensors; Oxygen; Scanning electron microscopy; Titanium dioxide; Electron transitions; glucose; glucose oxidase; iron phthalocyanine; metallophthalocyanine; multi walled nanotube; nanoparticle; nitrogen; phthalocyanine derivative; titanium dioxide nanoparticle; unclassified drug; amperometry; article; chemical reaction; cyclic potentiometry; direct electron transfer; electric conductivity; enzyme electrode; enzyme immobilization; enzyme stability; limit of detection; nanoanalysis; nanochemistry; oxidation kinetics; porosity; response time; scanning electron microscopy; sensitivity analysis; surface property; Aspergillus niger; Biosensing Techniques; Electrodes; Enzymes, Immobilized; Ferrous Compounds; Glucose; Glucose Oxidase; Indoles; Nanotubes, Carbon; Porosity; Sensitivity and Specificity; Titanium
AbstractGlucose oxidase (GOD) was adsorbed into a nanoporous TiO2 film layered on the surface of an iron phthalocyanine (FePc) vertically-aligned carbon nanotube (CNT) modified electrode. A Nafion film was then dropcast on the electrode's surface to improve operational and storage stabilities of the GOD-based electrode. Scanning electron microscopy (SEM) micrographs revealed the formation of FePc and nanoporous TiO2 nanoparticles along the sidewall and the tip of CNTs. Cyclic voltammograms of the GOD electrode in neutral PBS exhibited a pair of well-defined redox peaks, attesting the direct electron transfer of GOD (FAD/FADH2) with the underlying electrode. The potential of glucose electro-oxidation under nitrogen was ~+0.12V with an oxidation current density of 65.3μAcm-2 at +0.77V. Voltammetric and amperometric responses were virtually unaffected by oxygen, illustrating an efficient and fast direct electron transfer. The modification of the CNT surface with FePc resulted in a biosensor with remarkable detection sensitivity with an oxygen-independent bioelectrocatalysis. In deaerated PBS, the biosensor displayed average response time of 12s, linearity from 50μM to 4mM, and a detection limit of 30μM (S/N=3) for glucose. © 2013 Elsevier B.V.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270583
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Record identifierc66e7c67-a146-4dfe-bf21-872d11418657
Record created2014-02-17
Record modified2016-05-09
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