Structure of the LPS O-chain from Fusobacterium nucleatum strain 10953, containing sialic acid

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DOIResolve DOI: http://doi.org/10.1016/j.carres.2017.01.009
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TypeArticle
Journal titleCarbohydrate Research
ISSN0008-6215
Volume440-441
Pages3842
SubjectFusobacterium nucleatum; lipopolysaccharide; sialic acid
AbstractFusobacterium nucleatum is an anaerobic bacterium found in the human mouth where it causes periodontitis. Recently, it has been gaining attention as a potential causative agent for colorectal cancer and is strongly linked with pregnancy complications including pre-term and still births. Little is known about virulence factors of this organism and thus we have initiated studies to examine the bacterial surface glycochemistry. Consistent with a recent paper suggesting that F. nucleatum strain 10593 can synthesize sialic acid, a staining technique identified sialic acid on the bacterial surface. We isolated lipopolysaccharide from this F. nucleatum strain and performed structural analysis on the O-antigen. Our studies identified a trisaccharide repeating unit of the O-antigen with the following structure: -[→4)-α-Neup5Ac-(2 → 4)-β-d-Galp-(1 → 3)-α-d-FucpNAc4NAc-(1-]- where Ac indicates 4-N-acetylation of ∼30% FucNAc4N residues. The presence of sialic acid as a constituent of the O-antigen is consistent with recent data identifying de novo sialic acid synthesis in this strain.
Publication date
LanguageEnglish
AffiliationHuman Health Therapeutics; National Research Council Canada
Peer reviewedYes
NPARC number23001481
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Record identifierc66e99af-5337-492e-8f3f-7716ef0e2abd
Record created2017-02-17
Record modified2017-02-17
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