The genetic bases for the variation in the liposaccharide of the mucosal pathogen, Campylobacter jejuni : biosynthesis of sialylated ganglioside mimics in the core oligosaccharide

Download
  1. Get@NRC: The genetic bases for the variation in the liposaccharide of the mucosal pathogen, Campylobacter jejuni : biosynthesis of sialylated ganglioside mimics in the core oligosaccharide (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1074/jbc.M108452200
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleThe Journal of Biological Chemistry
Volume277
Issue1
Pages327337; # of pages: 11
AbstractWe have compared the lipo-oligosaccharide (LOS) biosynthesis loci from 11 Campylobacter jejunistrains expressing a total of 8 different ganglioside mimics in their LOS outer cores. Based on the organization of the genes, the 11 corresponding loci could be classified into three classes, with one of them being clearly an intermediate evolutionary step between the other two. Comparative genomics and expression of specific glycosyltransferases combined with in vitro activity assays allowed us to identify at least five distinct mechanisms that allowC. jejuni to vary the structure of the LOS outer core as follows: 1) different gene complements; 2) phase variation because of homopolymeric tracts; 3) gene inactivation by the deletion or insertion of a single base (without phase variation); 4) single mutation leading to the inactivation of a glycosyltransferase; and 5) single or multiple mutations leading to “allelic” glycosyltransferases with different acceptor specificities. The differences in the LOS outer core structures expressed by the 11 C. jejuni strains examined can be explained by one or more of the five mechanisms described in this work.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedYes
NPARC number19073093
Export citationExport as RIS
Report a correctionReport a correction
Record identifierc687e7b7-34cb-4f73-a1c1-fb88e1c28968
Record created2011-12-14
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)