Bacterial inhibition of methane clathrate hydrates formed in a stirred autoclave

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Journal titleEnergy & Fuels
Pages71707175; # of pages: 6
AbstractThe potential for hydrate inhibition in a stirred autoclave at a subcooling of 2.3 K has been tested on two bacterial isolates. Chryseobacterium sp. C14 survives multiple freeze–thaw events and inhibits ice recrystallization, and Escherichia coli has neither of these properties but is a biofilm producer. Both strains showed methane hydrate inhibition, with a significant reduction in total hydrate formed. Chryseobacterium delayed hydrate nucleating time, to a similar level found for the commercial kinetic inhibitor, polyvinylpyrrolidone (PVP; 0.2 wt %). However, in the presence of E. coli, in comparison to PVP, the time to nucleation was almost tripled and the hydrate growth rate was reduced by half. Because of the variation inherent with the microbial samples, likely a result of the complexities associated with varying cell numbers and metabolic activity, numerous experiments were required. Analysis of the distinct hydrate growth profiles shown by the two bacterial strains indicated that different molecular sources were likely responsible for the observed inhibition. As a working hypothesis, it is suggested that the inhibition observed by Chryseobacterium cultures was partially due to ice recrystallization inhibition properties, while that of E. coli may be due to secreted macromolecules and the effect of biofilm.
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AffiliationNRC Steacie Institute for Molecular Sciences; National Research Council Canada
Peer reviewedYes
NPARC number21268975
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Record identifierc7e2a981-e22a-4ea6-8212-1ca7dc372c8a
Record created2013-11-28
Record modified2016-05-09
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