Enzymatic synthesis of octyl glucoside catalyzed by almond β-glucosidase in organic media

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DOIResolve DOI: http://doi.org/10.1139/v02-081
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TypeArticle
Journal titleCanadian Journal of Chemistry
Volume80
Issue6
Pages653656; # of pages: 4
Subjectβ-glucosidase; alkyl glycosides; reverse hydrolysis; water activity; organic solvent; glycosides d'alkyles; hydrolyse inversée; activité de l'eau; solvant organique
AbstractThe synthesis of n-octyl-β-D-glucopyranoside can be performed by direct condensation of 1-octanol and glucose, catalyzed by immobilized almond β-glucosidase, using solid glucose suspended in 1-octanol as a reaction media. Both the rate of reaction and the conversion could be enhanced by using acetonitrile and N,N-dimethylformamide (DMF) as co-solvents, the latter giving the best results. The rate of reaction was dependent on the concentration of DMF and on the initial water activity (aw), with higher water activity fostering faster reactions. The rate increased with DMF concentration, up to 20% DMF, but diminished rapidly at higher concentrations. Product concentration could be increased from 40 to 100 mM by going from 0 to 20% DMF; however, it was not sensitive to the initial water activity.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NPARC number12338681
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Record identifierc7e9c3bc-205e-4521-aa1a-d9180b2187c3
Record created2009-09-10
Record modified2016-05-09
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