Overexpression of a FAD3 desaturase increases synthesis of a polymethylene-interrupted dienoic fatty acid in seeds of Arabidopsis thaliana L.

Download
  1. (PDF, 964 KB)
  2. Get@NRC: Overexpression of a FAD3 desaturase increases synthesis of a polymethylene-interrupted dienoic fatty acid in seeds of Arabidopsis thaliana L. (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1007/s11745-009-3315-5
AuthorSearch for: ; Search for: ; Search for: ; Search for:
TypeArticle
Journal titleLipids
Volume44
Issue8
Pages753757; # of pages: 5
SubjectLinoleate desaturase; Mangiferic acid; Polymethylene-interrupted dienoic fatty acid; Arabidopsis; Seed oil
AbstractA cDNA encoding the Arabidopsis extraplastidic linoleate desaturase (FAD3) was overexpressed in the seeds of wild-type Arabidopsis and in a mutant line that accumulates high levels of oleic acid. In the transformed wild-type plants, linolenic acid (18:3Δ9,12,15) increased from 19% to nearly 40% of total seed fatty acids, with a corresponding decrease in linoleate content (18:2Δ9,12). In the high oleate mutant, a large increase in the level of a fatty acid identified by gas-chromatography/mass-spectrometry as mangiferic acid (18:2Δ9,15) was observed. The results demonstrate that the polymethylene-interrupted dienoic fatty acid, mangiferic acid, can be produced in seed oil through the overexpression of a fatty acid n-3 desaturase.
Publication date
LanguageEnglish
AffiliationNRC Plant Biotechnology Institute; National Research Council Canada
Peer reviewedYes
NRC number50144
NPARC number19078543
Export citationExport as RIS
Report a correctionReport a correction
Record identifierc82b7e64-88c2-4831-b989-66ec2d59b778
Record created2012-03-30
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)