Enhancement of the antagonistic potency of transforming growth factor-beta receptor extracellular domains by coiled coil-induced homo- and heterodimerization

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DOIResolve DOI: http://doi.org/10.1074/jbc.M400655200
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TypeArticle
Journal titleThe Journal Of Biological Chemistry
Volume279
Issue25
Pages2601326018; # of pages: 6
AbstractTransforming growth factor-β (TGF-β) plays a causal role in several human pathologies including fibrotic diseases and metastasis. TGF-β signaling is mediated through its interaction with three types of cell surface receptors, RI, RII, and RIII. The soluble ectodomains of RII and RIII bind to TGF-β, making them attractive candidates to sequester TGF-β and inhibit its activity. To optimize the activity of the ectodomains, we studied the effect of artificially dimerizing them upon their kinetics of binding to TGF-β using an optical biosensor and studied their antagonistic potencies using an in vitro signaling assay. We fused the RII ectodomain and the membrane-proximal ligand-binding domain of the RIII ectodomain to de novo designed heterodimerizing coil strands and demonstrated that the coil strands within the fusion proteins were capable of promoting the dimerization of the coil-tagged ectodomains. Our results indicate that coiled coil-induced dimerization of the ectodomains stabilized their interaction with TGF-β as compared with the monomeric ectodomains. Also, in contrast to the monomeric ectodomains, which did not block signaling, the coiled coil-induced dimers were characterized by antagonistic potencies in the low nanomolar range.
Publication date
LanguageEnglish
AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NPARC number12338477
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Record identifierc8502932-5443-4a65-a474-ac900addee38
Record created2009-09-10
Record modified2016-05-09
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