Microbial diversity and activity in hypersaline high Arctic spring channels

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DOIResolve DOI: http://doi.org/10.1007/s00792-011-0417-9
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TypeArticle
Journal titleExtremophiles
Volume16
Issue2
Pages177191; # of pages: 15
SubjectCryomicrobiology; Arctic spring; Methane seep; Polar microbial ecology; Thaumarchaeota; Hypersaline
AbstractLost Hammer (LH) spring is a unique hypersaline, subzero, perennial high Arctic spring arising through thick permafrost. In the present study, the microbial and geochemical characteristics of the LH outflow channels, which remain unfrozen at ≥-18°C and are more aerobic/less reducing than the spring source were examined and compared to the previously characterized spring source environment. LH channel sediments contained greater microbial biomass (~100-fold) and greater microbial diversity reflected by the 16S rRNA clone libraries. Phylotypes related to methanogenesis, methanotrophy, sulfur reduction and oxidation were detected in the bacterial clone libraries while the archaeal community was dominated by phylotypes most closely related to THE ammonia-oxidizing Thaumarchaeota. The cumulative percent recovery of ¹⁴C-acetate mineralization in channel sediment microcosms exceeded ~30% and ~10% at 5 and -5°C, respectively, but sharply decreased at -10°C (≤1%). Most bacterial isolates (Marinobacter, Planococcus, and Nesterenkonia spp.) were psychrotrophic, halotolerant, and capable of growth at -5°C. Overall, the hypersaline, subzero LH spring channel has higher microbial diversity and activity than the source, and supports a variety of niches reflecting the more dynamic and heterogeneous channel environment.
Publication date
LanguageEnglish
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC number53407
NPARC number19697992
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Record identifierc9451f13-1b16-4661-9131-d73dfb411e01
Record created2012-05-18
Record modified2016-05-09
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