Mapping the myoglobin concentration, oxygenation, and optical pathlength in heart ex vivo using near-infrared imaging

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DOIResolve DOI: http://doi.org/10.1016/j.ab.2010.07.010
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TypeArticle
Journal titleAnalytical Biochemistry
Volume407
Issue1
Pages120127; # of pages: 8
SubjectMyoglobin; NIR light diffuse reflectance; NIR spectroscopic imaging; First derivative; Optical pathlength; Pig heart
AbstractA method that provides maps of absolute concentrations of oxygenated and deoxygenated myoglobin (Mb), its oxygenation, and its near-infrared (NIR) optical pathlength in cardiac tissue was developed. These parameters are available simultaneously. The method is based on NIR diffuse reflectance spectroscopic imaging and specific processing of the NIR images, which included a first derivative of the diffuse reflectance spectrum. Mb oxygenation, total Mb concentration, and NIR light pathlength were found to be in the range of 92%, 0.3 mM, and 12.5 mm, respectively, in beating isolated buffer-perfused and arrested pig hearts. The charge-coupled device camera enables sub-millimeter spatial resolution and spectroscopic imaging in 1.5 to 2.0 min. The technique is noninvasive and nondestructive. The equipment has no mechanical contact with the tissue of interest, leaving it undisturbed.
Publication date
LanguageEnglish
AffiliationNRC Institute for Biodiagnostics; National Research Council Canada
Peer reviewedYes
NPARC number17673456
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Record identifierc9e006ef-2150-4b49-8541-a232f3291cc0
Record created2011-04-03
Record modified2016-05-09
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